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reporter plasmids mutant pglo-car10  (GenScript corporation)

 
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    Structured Review

    GenScript corporation reporter plasmids mutant pglo-car10
    CAR10 is the target gene of miR-125b-5p. (a, b) The effect of CAR10 overexpression on miR-125b-5p, miR-1249, miR-2277-5p, miR-3192, miR-3663-5p, and miR-3692-5p expression level was detected by RT-qPCR in C33A and HeLa cells, # p < 0.05, compared with pcDNA-NC. (c) Detection of miR-125b-5p expression levels in cervical cancer tissues and paracancerous tissues collected by RT-qPCR, # p < 0.05, compared with adjacent controls. (d) The miR-125b-5p inhibitor (anti-miR-125b-5p) and the negative control Mock were transfected into C33A and HeLa, and the expression level of miR-125b-5p was detected by RT-qPCR, # p < 0.05, compared with Mock. (e, f) The effect of anti-miR-125b-5p on the activity of pGLO-CAR10 WT and pGLO-CAR10 Mut was examined by <t>luciferase</t> assay, # p < 0.05, compared with Mock. (g, h) The interaction of miR-125b-5p and CAR10 was detected by Ago2-RIP-qPCR assay, # p < 0.05, compared with IgG.
    Reporter Plasmids Mutant Pglo Car10, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reporter+plasmids+mutant+pglo-car10/pmc06984746-70-0-19?v=GenScript+corporation
    Average 90 stars, based on 1 article reviews
    reporter plasmids mutant pglo-car10 - by Bioz Stars, 2026-07
    90/100 stars

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    1) Product Images from "LncRNA CAR10 Upregulates PDPK1 to Promote Cervical Cancer Development by Sponging miR-125b-5p"

    Article Title: LncRNA CAR10 Upregulates PDPK1 to Promote Cervical Cancer Development by Sponging miR-125b-5p

    Journal: BioMed Research International

    doi: 10.1155/2020/4351671

    CAR10 is the target gene of miR-125b-5p. (a, b) The effect of CAR10 overexpression on miR-125b-5p, miR-1249, miR-2277-5p, miR-3192, miR-3663-5p, and miR-3692-5p expression level was detected by RT-qPCR in C33A and HeLa cells, # p < 0.05, compared with pcDNA-NC. (c) Detection of miR-125b-5p expression levels in cervical cancer tissues and paracancerous tissues collected by RT-qPCR, # p < 0.05, compared with adjacent controls. (d) The miR-125b-5p inhibitor (anti-miR-125b-5p) and the negative control Mock were transfected into C33A and HeLa, and the expression level of miR-125b-5p was detected by RT-qPCR, # p < 0.05, compared with Mock. (e, f) The effect of anti-miR-125b-5p on the activity of pGLO-CAR10 WT and pGLO-CAR10 Mut was examined by luciferase assay, # p < 0.05, compared with Mock. (g, h) The interaction of miR-125b-5p and CAR10 was detected by Ago2-RIP-qPCR assay, # p < 0.05, compared with IgG.
    Figure Legend Snippet: CAR10 is the target gene of miR-125b-5p. (a, b) The effect of CAR10 overexpression on miR-125b-5p, miR-1249, miR-2277-5p, miR-3192, miR-3663-5p, and miR-3692-5p expression level was detected by RT-qPCR in C33A and HeLa cells, # p < 0.05, compared with pcDNA-NC. (c) Detection of miR-125b-5p expression levels in cervical cancer tissues and paracancerous tissues collected by RT-qPCR, # p < 0.05, compared with adjacent controls. (d) The miR-125b-5p inhibitor (anti-miR-125b-5p) and the negative control Mock were transfected into C33A and HeLa, and the expression level of miR-125b-5p was detected by RT-qPCR, # p < 0.05, compared with Mock. (e, f) The effect of anti-miR-125b-5p on the activity of pGLO-CAR10 WT and pGLO-CAR10 Mut was examined by luciferase assay, # p < 0.05, compared with Mock. (g, h) The interaction of miR-125b-5p and CAR10 was detected by Ago2-RIP-qPCR assay, # p < 0.05, compared with IgG.

    Techniques Used: Over Expression, Expressing, Quantitative RT-PCR, Negative Control, Transfection, Activity Assay, Luciferase

    CAR10 acted as a ceRNA and upregulated the expression of PDPK1 by sponging miR-125b-5p. (a) The binding site of miR-125b-5p to the 3′UTR of PDPK1 was predicted by bioinformatics, and the binding site was mutated to construct luciferase reporter gene plasmids. (b, c) The effect of anti-miR-125b-5p on the activity of PDPK1 3′UTR WT and PDPK1 3′ UTR Mut was examined by luciferase assay in C33A and HeLa cells, # p < 0.05, compared with Mock. (d, e, f) The effects of anti-miR-125b-5p on the expression of PDPK1 mRNA and protein were detected by RT-qPCR and western blot, # p < 0.05, compared with Mock. (g) The mRNA expression level of PDPK1 was detected by RT-qPCR in the collected cervical cancer tissues and adjacent tissues, # p < 0.05, compared with adjacent controls. The miR-125b-5p mimic (miR-125b-5p) and the negative control Mock were synthesized and transfected into C33A and HeLa cells, and (h) the expression level of miR-125b-5p was detected by RT-qPCR, # p < 0.05, compared with Mock. (i, j) The effect of miR-125b-5p and/or CAR10 overexpression on the activity of PDPK1 3′UTR WT and PDPK1 3′ UTR Mut was examined by luciferase assay, # p < 0.05, compared with CON. Ф p < 0.05, compared with CAR10 + miR-125b-5p. (k) The effect of miR-125b-5p and/or CAR10 overexpression on PDPK1 expression was observed by western blot and RT-qPCR, # p < 0.05, compared with CON. Ф p < 0.05, compared with CAR10 + miR-125b-5p.
    Figure Legend Snippet: CAR10 acted as a ceRNA and upregulated the expression of PDPK1 by sponging miR-125b-5p. (a) The binding site of miR-125b-5p to the 3′UTR of PDPK1 was predicted by bioinformatics, and the binding site was mutated to construct luciferase reporter gene plasmids. (b, c) The effect of anti-miR-125b-5p on the activity of PDPK1 3′UTR WT and PDPK1 3′ UTR Mut was examined by luciferase assay in C33A and HeLa cells, # p < 0.05, compared with Mock. (d, e, f) The effects of anti-miR-125b-5p on the expression of PDPK1 mRNA and protein were detected by RT-qPCR and western blot, # p < 0.05, compared with Mock. (g) The mRNA expression level of PDPK1 was detected by RT-qPCR in the collected cervical cancer tissues and adjacent tissues, # p < 0.05, compared with adjacent controls. The miR-125b-5p mimic (miR-125b-5p) and the negative control Mock were synthesized and transfected into C33A and HeLa cells, and (h) the expression level of miR-125b-5p was detected by RT-qPCR, # p < 0.05, compared with Mock. (i, j) The effect of miR-125b-5p and/or CAR10 overexpression on the activity of PDPK1 3′UTR WT and PDPK1 3′ UTR Mut was examined by luciferase assay, # p < 0.05, compared with CON. Ф p < 0.05, compared with CAR10 + miR-125b-5p. (k) The effect of miR-125b-5p and/or CAR10 overexpression on PDPK1 expression was observed by western blot and RT-qPCR, # p < 0.05, compared with CON. Ф p < 0.05, compared with CAR10 + miR-125b-5p.

    Techniques Used: Expressing, Binding Assay, Construct, Luciferase, Activity Assay, Quantitative RT-PCR, Western Blot, Negative Control, Synthesized, Transfection, Over Expression



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    reporter plasmids mutant pglo-car10  (GenScript corporation)
    90
    GenScript corporation reporter plasmids mutant pglo-car10
    CAR10 is the target gene of miR-125b-5p. (a, b) The effect of CAR10 overexpression on miR-125b-5p, miR-1249, miR-2277-5p, miR-3192, miR-3663-5p, and miR-3692-5p expression level was detected by RT-qPCR in C33A and HeLa cells, # p < 0.05, compared with pcDNA-NC. (c) Detection of miR-125b-5p expression levels in cervical cancer tissues and paracancerous tissues collected by RT-qPCR, # p < 0.05, compared with adjacent controls. (d) The miR-125b-5p inhibitor (anti-miR-125b-5p) and the negative control Mock were transfected into C33A and HeLa, and the expression level of miR-125b-5p was detected by RT-qPCR, # p < 0.05, compared with Mock. (e, f) The effect of anti-miR-125b-5p on the activity of pGLO-CAR10 WT and pGLO-CAR10 Mut was examined by <t>luciferase</t> assay, # p < 0.05, compared with Mock. (g, h) The interaction of miR-125b-5p and CAR10 was detected by Ago2-RIP-qPCR assay, # p < 0.05, compared with IgG.
    Reporter Plasmids Mutant Pglo Car10, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reporter+plasmids+mutant+pglo-car10/pmc06984746-70-0-19?v=GenScript+corporation
    Average 90 stars, based on 1 article reviews
    reporter plasmids mutant pglo-car10 - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    CAR10 is the target gene of miR-125b-5p. (a, b) The effect of CAR10 overexpression on miR-125b-5p, miR-1249, miR-2277-5p, miR-3192, miR-3663-5p, and miR-3692-5p expression level was detected by RT-qPCR in C33A and HeLa cells, # p < 0.05, compared with pcDNA-NC. (c) Detection of miR-125b-5p expression levels in cervical cancer tissues and paracancerous tissues collected by RT-qPCR, # p < 0.05, compared with adjacent controls. (d) The miR-125b-5p inhibitor (anti-miR-125b-5p) and the negative control Mock were transfected into C33A and HeLa, and the expression level of miR-125b-5p was detected by RT-qPCR, # p < 0.05, compared with Mock. (e, f) The effect of anti-miR-125b-5p on the activity of pGLO-CAR10 WT and pGLO-CAR10 Mut was examined by luciferase assay, # p < 0.05, compared with Mock. (g, h) The interaction of miR-125b-5p and CAR10 was detected by Ago2-RIP-qPCR assay, # p < 0.05, compared with IgG.

    Journal: BioMed Research International

    Article Title: LncRNA CAR10 Upregulates PDPK1 to Promote Cervical Cancer Development by Sponging miR-125b-5p

    doi: 10.1155/2020/4351671

    Figure Lengend Snippet: CAR10 is the target gene of miR-125b-5p. (a, b) The effect of CAR10 overexpression on miR-125b-5p, miR-1249, miR-2277-5p, miR-3192, miR-3663-5p, and miR-3692-5p expression level was detected by RT-qPCR in C33A and HeLa cells, # p < 0.05, compared with pcDNA-NC. (c) Detection of miR-125b-5p expression levels in cervical cancer tissues and paracancerous tissues collected by RT-qPCR, # p < 0.05, compared with adjacent controls. (d) The miR-125b-5p inhibitor (anti-miR-125b-5p) and the negative control Mock were transfected into C33A and HeLa, and the expression level of miR-125b-5p was detected by RT-qPCR, # p < 0.05, compared with Mock. (e, f) The effect of anti-miR-125b-5p on the activity of pGLO-CAR10 WT and pGLO-CAR10 Mut was examined by luciferase assay, # p < 0.05, compared with Mock. (g, h) The interaction of miR-125b-5p and CAR10 was detected by Ago2-RIP-qPCR assay, # p < 0.05, compared with IgG.

    Article Snippet: Luciferase reporter plasmids include wild-type and mutant pGLO-CAR10, as well as wild-type and mutant pGLO-PDPK1 3′UTR, both constructed by GenScript Company (Nanjing, China).

    Techniques: Over Expression, Expressing, Quantitative RT-PCR, Negative Control, Transfection, Activity Assay, Luciferase

    CAR10 acted as a ceRNA and upregulated the expression of PDPK1 by sponging miR-125b-5p. (a) The binding site of miR-125b-5p to the 3′UTR of PDPK1 was predicted by bioinformatics, and the binding site was mutated to construct luciferase reporter gene plasmids. (b, c) The effect of anti-miR-125b-5p on the activity of PDPK1 3′UTR WT and PDPK1 3′ UTR Mut was examined by luciferase assay in C33A and HeLa cells, # p < 0.05, compared with Mock. (d, e, f) The effects of anti-miR-125b-5p on the expression of PDPK1 mRNA and protein were detected by RT-qPCR and western blot, # p < 0.05, compared with Mock. (g) The mRNA expression level of PDPK1 was detected by RT-qPCR in the collected cervical cancer tissues and adjacent tissues, # p < 0.05, compared with adjacent controls. The miR-125b-5p mimic (miR-125b-5p) and the negative control Mock were synthesized and transfected into C33A and HeLa cells, and (h) the expression level of miR-125b-5p was detected by RT-qPCR, # p < 0.05, compared with Mock. (i, j) The effect of miR-125b-5p and/or CAR10 overexpression on the activity of PDPK1 3′UTR WT and PDPK1 3′ UTR Mut was examined by luciferase assay, # p < 0.05, compared with CON. Ф p < 0.05, compared with CAR10 + miR-125b-5p. (k) The effect of miR-125b-5p and/or CAR10 overexpression on PDPK1 expression was observed by western blot and RT-qPCR, # p < 0.05, compared with CON. Ф p < 0.05, compared with CAR10 + miR-125b-5p.

    Journal: BioMed Research International

    Article Title: LncRNA CAR10 Upregulates PDPK1 to Promote Cervical Cancer Development by Sponging miR-125b-5p

    doi: 10.1155/2020/4351671

    Figure Lengend Snippet: CAR10 acted as a ceRNA and upregulated the expression of PDPK1 by sponging miR-125b-5p. (a) The binding site of miR-125b-5p to the 3′UTR of PDPK1 was predicted by bioinformatics, and the binding site was mutated to construct luciferase reporter gene plasmids. (b, c) The effect of anti-miR-125b-5p on the activity of PDPK1 3′UTR WT and PDPK1 3′ UTR Mut was examined by luciferase assay in C33A and HeLa cells, # p < 0.05, compared with Mock. (d, e, f) The effects of anti-miR-125b-5p on the expression of PDPK1 mRNA and protein were detected by RT-qPCR and western blot, # p < 0.05, compared with Mock. (g) The mRNA expression level of PDPK1 was detected by RT-qPCR in the collected cervical cancer tissues and adjacent tissues, # p < 0.05, compared with adjacent controls. The miR-125b-5p mimic (miR-125b-5p) and the negative control Mock were synthesized and transfected into C33A and HeLa cells, and (h) the expression level of miR-125b-5p was detected by RT-qPCR, # p < 0.05, compared with Mock. (i, j) The effect of miR-125b-5p and/or CAR10 overexpression on the activity of PDPK1 3′UTR WT and PDPK1 3′ UTR Mut was examined by luciferase assay, # p < 0.05, compared with CON. Ф p < 0.05, compared with CAR10 + miR-125b-5p. (k) The effect of miR-125b-5p and/or CAR10 overexpression on PDPK1 expression was observed by western blot and RT-qPCR, # p < 0.05, compared with CON. Ф p < 0.05, compared with CAR10 + miR-125b-5p.

    Article Snippet: Luciferase reporter plasmids include wild-type and mutant pGLO-CAR10, as well as wild-type and mutant pGLO-PDPK1 3′UTR, both constructed by GenScript Company (Nanjing, China).

    Techniques: Expressing, Binding Assay, Construct, Luciferase, Activity Assay, Quantitative RT-PCR, Western Blot, Negative Control, Synthesized, Transfection, Over Expression